Purifying Alpha Amylase from Aspergillus Niger

Presenter: Mercy Retana

Co-Presenter(s):
Jamie Brooks

Presenter Status: Undergraduate student

Academic Year: 22-23

Semester: Spring

Faculty Mentor: Monica Lares

Department: Chemistry

Funding Source/Sponsor: Class Project

President's Strategic Plan Goal: Connectivity and Community Engagement

Abstract:
We extracted and purified the enzyme Alpha Amylase from Asergillus Niger. First was did Solid State Fermentation with orange peels as a substrate. We centrifuged and the precipitated sample under went dialysis, and purification using DEAE-Sephacel for column chromatography and SDS Page. We also performed a DNS Assay to test for enzyme activity.