Extraction and Purification

Extraction and Purification of Superoxide Dismutase From Allium sativum

Presenter: Kelsey Edgar

Co-Presenter(s):
Abigail Cabrera Padilla

Presenter Status: Undergraduate student

Department: Chemistry

Screenshot URL: https://drive.google.com/uc?id=1cxV8Gvyr9uCgiNqGNP7m3dlGSM1S93cR

Abstract:
This research aims to investigate the presence of Cu,Zn-Superoxide Dismutase (SOD) in fresh garlic through the purification process of ammonium sulfate precipitation, ion exchange batching, and size exclusion column chromatography. SOD is an important enzyme of organisms because it is the first line of defense against oxidative stress. Cu,Zn-SOD is the most predominant SOD in plant tissue and it has been purified from a variety of plant sources. For this research garlic (Allium sativum L.) was decided to be the extraction source for Cu,Zn-SOD since it is the plant that contains the most abundant. Determining the activity of SOD was done by utilizing the pyrogallol method primarily because it was developed specifically to measure the superoxide-scavenging ability of SOD. In this study, the best conditions were determined to test the activity of SOD extracted from garlic.