Chromosomal organization in renal carcinoma cell lines

Chromosomal organization in primary and secondary clear cell renal cell carcinoma

Presenter: Fezza Arshad

Co-Presenter(s):
Lexie Haralson

Presenter Status: Graduate student

Academic Year: 22-23

Semester: Spring

Department: Biology

Funding Source/Sponsor: Koret Scholars Program

President's Strategic Plan Goal: Adaptability and Responsiveness

Screenshot URL: https://drive.google.com/uc?id=1v6Kz6QMoU9wqAzojXBg1Zu5wVk3Axit6

Abstract:
Abstract: Currently, there is a paucity of information for chromosomal organization in primary and metastatic tumors. Primary and metastatic tumors are heterogeneous cell populations with genetic instabilities. Recently, it was shown that individual homologous chromosomes are spatially segregated, or antipaired, during mitosis in normal human cells. The antipairing pattern was lost for chromosome 19 in a secondary/metastatic clear cell renal cell carcinoma (ccRCC) cell line, Caki-1. Genes on chromosome 19 have been shown to be mis-regulated in Caki-1 cells suggesting that the antipairing mechanism may function to prevent abnormal homologous pairing, and gene mis-regulation. However, it is unknown whether the loss of antipairing pattern is present prior to metastasis. To test whether the loss of mitotic antipairing occurs prior to metastasis in a ccRCC, we performed chromosome painting of chromosome 19 for a primary ccRCC cell line, 786-O. Using high resolution confocal microscopy, and 3D reconstruction, we found that the loss of antipairing is not observed in primary renal carcinoma cells. The data suggests that the loss of antipairing may be a unique characteristic of secondary/metastatic tumors with increased genetic instability. Findings of our study will aid in elucidating mechanisms of tumorigenesis, and shed light on efficacies of tumor response to immunotherapies.