Presenter
:
Christian LeRue
Co-Presenter(s)
:
Blaine Baker, Thomas Rodriguez
Presenter Status
:
Undergraduate student
Academic Year
:
20-21
Semester
:
Spring
Faculty Mentor
:
Monica Lares
Department
:
Chemistry
Funding Source/Program
:
Class Project
Presentation Link
:
Abstract
:
Previous studies have shown a strong correlation between peroxide concentration and activity in plants and their ability to stay fresh and ultimately stay nutrient rich [1]. In this experiment, a correlation was determined between the fridge age of broccoli and the corresponding peroxidase activity in the broccoli stems. Broccoli peroxidase was purified from broccoli stems via standard protein purification methods: Ammonium sulfate precipitation, dialyzing membranes, and size exclusion chromatography. The concentration and activity of Peroxidase was determined via the Bradford Assay, the highly specific Guaiacol assay, and an SDS-PAGE to verify the molecular weight. Kinetic parameters such as Km for Guaiacol were determined