Bioremediation of Lipids

Bioremediation of Lipids Using Organic Lipase

Presenter: Clarissa Alcala

Co-Presenter(s):
George Darwood

Presenter Status: Undergraduate student

Academic Year: 19-20

Semester: Spring

Faculty Mentor: Monica Lares

Department: Chemistry

Screenshot URL: https://drive.google.com/uc?id=1HSXYlh8tI4ulGPtNnXJtKo9Wzj1VAf_x

Abstract:
Using orange peels and 7.5 pH Tris-HCl buffer, the enzyme lipase was extracted and purified using the methods of ammonium sulfate precipitation and size exclusion column chromatography, in order to determine if organic lipase can be used in the bioremediation of lipids, produced by cooking oil waste.  Theoretically, lipase can catalyze the hydrolysis of fats in vitro and thus can be used to break down fats and lipids outside of the body, potentially to be useful for remediating oil spills and for easy disposal of cooking oil waste.