Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao) Flowers

Students: Lucas Weston, Hammad Nagda

Faculty Mentor: Monica Lares

Chemistry

College of Science, Technology, and Business

The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of Musa spp. (Baxijiao). Using NMR spectroscopy analysis, they were identified as vanillic acid (1), ferulic acid (2), β-sitosterol (3), daucosterol (4), and 9-(4′-hydroxyphenyl)-2-methoxyphenalen-1-one (5). The half-maximal inhibitory concentration (IC50) values of compounds 1–5 were 2004.58, 1258.35, 283.67, 247.35, and 3.86 mg/L, respectively. Compared to a known α-glucosidase inhibitor (acarbose, IC50 = 999.31 mg/L), compounds 3, 4, and 5 showed a strong α-glucosidase inhibitory effect. A Lineweaver-Burk plot indicated that compound 5 is a mixed-competitive inhibitor, while compounds 3 and 4 are competitive inhibitors. The inhibition constants (Ki) of compounds 3, 4, and 5 were 20.09, 2.34, and 4.40 mg/L, respectively. Taken together, these data show that compounds 3, 4, and 5 are potent α-glucosidase inhibitors.